Protein Engineering

(Department)  Biomedical Engineering         (Division)      Biomaterials and Tissue Engineering
 
 (Level and Major)   Graduate
 

Course Title                Protein Engineering              
 
Number of Credits       3             Prerequisite


Course Description:
Protein engineering describes methods of changing the protein sequence to achieve the desired result for a specific application.
 
Course Goals and Objectives:
The aim of the course is to study the techniques in which modify a protein sequence by substituting, inserting, or deleting nucleotides into an encoding gene, with the goal of obtaining a modified protein that is more suitable than an unmodified protein for a specific application or purpose. 
 
Course Topics:
  • Introduction to protein engineering
  • Structures and conformations of proteins
  • Expression of proteins
  • Protein sequencing and purification
  • DNA Sequencing
  • Natural mutagenesis and recombination
  • Vectors
  • Recombinant DNA technology  and cloning
  • Engineering of antibodies, enzymes
  • Applications of recombinant proteins in tissue engineering and biomaterials
  • Computer methods in protein modeling
  • In vivo and in vitro tests for the detection and evaluation of proteins
 
The course aims to:
  • describe the structure and classification of proteins
  • describe the methods of protein recombination
  • structure of proteins, and relate this information to the function of proteins
  • explain how proteins can be used for different industrial and academic purposes
  • Explain the applications of recombinant proteins in tissue engineering and biomaterials
  • design primers to introduce mutations by means of PCR
 
Reading Resources:
  • T.A., gene Cloning and DNA Analysis: An Introduction, 7th ed, 2016, Wiley-Blackwell.
  • Jeremy B., Tymoczko J., Stryer L., Biochemistry. 5th ed., 2006, New York, NY: W.H. Freeman and Company.
  • Miller M., Arndt M., Arndtk, Protein Engineering Protocols (Methods in Molecular Biology), 2006, Humana Press.
  • Lutz S., Bornscheuer U., Protein Engineering Handbook, 2006, Wiley-VCH.
  • Alberghina L., Protein Engineering for Industrial Biotechnology, 2000, CRC.
  • Twyman R.M., Principles of Proteomics (Advanced Text Series), 2004, Bias Scientific Publication.         
 
Evaluation
Homework, term projects, midterm and final exam.

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